A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.

ABSTRACT: The ability to manipulate gene activity and control transgene expression is essential to study gene function. While several genetic tools for modifying genes or controlling expression separately are available for Caenorhabditis elegans, there are no genetic approaches to generate mutations that simultaneously disrupt gene function and provide genetic access to the cells expressing the disrupted gene. To achieve this, we developed a versatile gene trap strategy based on cGAL, a GAL4-UAS bipartite expression system for C. elegans. We designed a cGAL gene trap cassette and used CRISPR/Cas9 to insert it into the target gene, creating a bicistronic operon that simultaneously expresses a truncated endogenous protein and the cGAL driver in the cells expressing the target gene. We demonstrate that

SUBMITTER: Khan H 

PROVIDER: S-EPMC11753634 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature