Influences of pulsed electric field parameters on cell electroporation and electrofusion events: Comprehensive understanding by experiments and molecular dynamics simulations.

ABSTRACT: Electroporation and electrofusion are efficient methods, which have been widely used in different areas of biotechnology and medicine. Pulse strength and width, as an external condition, play an important role in the process of these methods. However, comparatively little work has been done to explore the effects of pulsed electric field parameters on electroporation and electrofusion. Herein, influences of pulse strength and width on the electroporation and electrofusion of phospholipid bilayers were systematically investigated by using experiments combined with molecular dynamics simulations. Experimental results and machine learning-based regression analysis showed that the number of pores is mainly determined by pulse strength, while the sizes of pores were enlarged by increasing the p

SUBMITTER: Qu S 

PROVIDER: S-EPMC11753653 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature