HMGB1 lactylation drives neutrophil extracellular trap formation in lactate-induced acute kidney injury.

ABSTRACT:

Rationale

Acute kidney injury (AKI) is a clinical syndrome associated with a multitude of conditions. Although renal replacement therapy (RRT) remains the cornerstone of treatment for advanced AKI, its implementation can potentially pose risks and may not be readily accessible across all healthcare settings and regions. Elevated lactate levels are implicated in sepsis-induced AKI; however, it remains unclear whether increased lactate directly induces AKI or elucidates the underlying mechanisms.

Methods

For human, the measurement of lactate in arterial blood gas is performed using the direct determination of L-lactate through an electrode oxidation method by a blood gas analyzer. For mice, enzyme-linked immunosorbent assay (ELISA) kits were employed to quantify the concentrat

SUBMITTER: Zhu L 

PROVIDER: S-EPMC11754054 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature