Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis <i>in vitro</i> and <i>in vivo</i>.

ABSTRACT:

Object

Neuroinflammation mediated by microglia has emerged as a critical factor in ischemic stroke and neuronal damage. Gualou Guizhi Granule (GLGZG) has been shown to suppress inflammation in lipopolysaccharide (LPS)-activated microglia, though the underlying mechanisms and its protective effects against neuronal apoptosis remain unclear. This study aims to investigate how GLGZG regulates the Notch signaling pathway in microglia to reduce neuroinflammation and protect neurons from apoptosis.

Method

Using in vitro and in vivo models, we explored GLGZG's impact on microglia activation, pro-inflammatory cytokines, and neuronal apoptosis. Microglial cells were activated with LPS, and primary neuronal cells were exposed to LPS-activated microglia to simulate neuroi

SUBMITTER: Li X 

PROVIDER: S-EPMC11754197 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature