Circle-seq reveals that eccDNA may be a key blood biomarker for HBV-associated liver cancer.

ABSTRACT:

Introduction

Extrachromosomal circular DNA (eccDNA) regulates tumor occurrence and development. Relevant eccDNA profiles have been established for various types of cancer; however, the eccDNA expression profiles in the blood of patients with hepatocellular carcinoma (HCC) and liver cirrhosis (LC) remain unknown. The present study aimed to investigate the eccDNA expression profiles in the blood of patients with HCC and LC.

Methods

Circle-seq was used to detect eccDNAs in the blood samples. Full transcript sequencing was used to analyze the RNA in the samples. Geno Ontology enrichment and Kyoto Encyclopedia of Genes and Genome pathway analyses were performed on differentially expressed eccDNA-related genes. The identified eccDNA is combined with mRNA to screen target genes usi

SUBMITTER: Ran XK 

PROVIDER: S-EPMC11754267 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature