Optimization of canine sperm cryopreservation by focusing on glycerol concentration and freezing rate.

ABSTRACT: The purpose of this study was to improve the quality of frozen-thawed canine spermatozoa through the optimization of glycerol concentration (GC) and freezing rate in the semen freezing protocol. Ejaculates from nine dogs were diluted with an extender containing 0%, 1.5%, 3%, 6%, or 9% glycerol. The suspensions were loaded into 0.25 ml straws, frozen in nitrogen vapor in a closed box, and immersed in liquid nitrogen (LN₂). The freezing rate was controlled by setting the distance from the LN₂ surface to the straws as 1, 4, 7, or 10 cm. Firstly, freezing curves for each GC and freezing rate were analyzed. The analysis showed that the temperature of ice nucleation, freezing point, and immersion were changed with a certain trend depending on the GCs and freezing rates. Sec

SUBMITTER: Ogata K 

PROVIDER: S-EPMC11754312 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature