Identification of a novel butenolide signal system to regulate high production of tylosin in Streptomyces fradiae.

ABSTRACT: Identifying hormone-like quorum sensing (QS) molecules in streptomycetes is challenging due to low production levels but is essential for understanding secondary metabolite biosynthesis and morphological differentiation. This work reports the discovery of a novel γ-butenolide-type signaling molecule (SFB1) via overexpressing its biosynthetic gene (orf18) in Streptomyces fradiae. SFB1 was found to be essential for production of tylosin through dissociating the binding of its receptor TylP (a transcriptional repressor) to target genes, thus activating the expression of tylosin biosynthetic gene cluster (tyl). Meanwhile, SFB1 biosynthesis is negatively regulated by TylQ (another transcriptional repressor); the disruption of its coding gene tylQ led to increased production of SFB1, which in tu

SUBMITTER: Chen H 

PROVIDER: S-EPMC11754346 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature