Irradiation alters extracellular vesicle microRNA load in the serum of patients with leukaemia.

ABSTRACT:

Purpose

Recent data suggest an impact of extracellular vesicles (EVs) and their micro(mi)RNA cargo on cell-cell interactions to contribute to pathophysiology of leukaemia and radiation response. Here, we investigated differential miRNA cargo of EVs from serum derived from patients with leukaemia (n = 11) before and after total body irradiation with 2 × 2 Gy as compared to healthy donors (n = 6).

Methods

RNA was isolated from EVs and subjected to next generation sequencing of miRNAs. Analysis of sequencing data was performed with miRDeep29 software and differentially expressed miRNAs were filtered using R package edgeR10,11. Signaling pathways were identified using Kyoto Encyclopedia of Genes and Genomes database (KEGG) pathway analysis.

Results

Flow cytometric and Wes

SUBMITTER: Hehlgans S 

PROVIDER: S-EPMC11754379 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature