<i>Streptococcus intermedius</i> promotes synchronous multiple primary lung cancer progression through apoptosis regulation.

ABSTRACT:

Background

Dysbiosis of the lung microbiome can contribute to the initiation and progression of lung cancer. Synchronous multiple primary lung cancer (sMPLC) is an increasingly recognized subtype of lung cancer characterized by high morbidity, difficulties in early detection, poor prognosis, and substantial clinical challenges. However, the relationship between sMPLC pathogenesis and changes in the lung microbiome remains unclear.

Methods

In this study, 16S rRNA sequencing was performed on clinical samples to analyze lung microbiome composition. Real-time quantitative PCR (qPCR) was used to quantify bacterial abundance in lung tissues. In addition, flow cytometry was conducted to evaluate cell cycle progression and apoptosis in lung tumor cells.

Results

Clinical cohor

SUBMITTER: Deng Y 

PROVIDER: S-EPMC11754412 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature