A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans.

ABSTRACT: Lectins are produced in almost all life forms, can interact with targets (glycans) in a cross-kingdom manner and have served as valuable tools for studying glycobiology. Previously, a bacterial lectin, named Streptomyces hemagglutinin (SHA), was found to agglutinate human type B erythrocytes. However, the binding of SHA to mammalian cell types other than human erythrocytes has not been explored. To address this, we produced a recombinant fusion protein, with the mCherry reporter protein proceeding the SHA protein (referred to as mCherry-SHA), and performed co-immunofluorescence staining analysis. We focused on the normal pancreas in this study because glycans on pancreatic cells have been associated with initiation and progression of pancreatic cancer, a deadly disease. We found that only

SUBMITTER: Quijano JC 

PROVIDER: S-EPMC11754455 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature