Sensitive ratiometric detection of Fumonisin B₁ using a reusable Ag-pSi SERS platform.

ABSTRACT: Food and agricultural commodities endure consistent contamination by mycotoxins, low molecular weight fungal metabolites, which pose severe health implications to humans together with staggering economic losses. Herein, a ratiometric aptasensor was constructed using silver-coated porous silicon (Ag-pSi) used as an efficient surface-enhanced Raman scattering (SERS) substrate. The bioassay included direct detection of fumonisin B₁ (FB₁), an abundant and widespread contaminant, by a specific aptamer sequence immobilized on the porous transducer. The inherent surface void and pore morphology were physically optimized to achieve a sufficient SERS effect (enhancement factor > 5 × 10⁷). Under optimal conditions, the aptasensor exhibits high sensitivity, wide dynam

SUBMITTER: Nirala NR 

PROVIDER: S-EPMC11754687 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature