Motif distribution and DNA methylation underlie distinct Cdx2 binding during development and homeostasis.

ABSTRACT: Transcription factors guide tissue development by binding to developmental stage-specific targets and establishing an appropriate enhancer landscape. In turn, DNA and chromatin modifications direct the genomic binding of transcription factors. However, how transcription factors navigate chromatin features to selectively bind a small subset of all the possible genomic target loci remains poorly understood. Here we show that Cdx2-a lineage defining transcription factor that binds distinct targets in developing versus adult intestinal epithelial cells-has a preferential affinity for a non-canonical CpG-containing motif in vivo. A higher frequency of this motif at embryonic Cdx2 targets and methylated state of the CpG during development enables selective Cdx2 binding and activation of developm

SUBMITTER: Lorzadeh A 

PROVIDER: S-EPMC11754732 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature