Linkage Mapping and Identification of Candidate Genes for Cold Tolerance in Rice (Oryza Sativa L.) at the Bud Bursting Stage.

ABSTRACT: Rice is highly sensitive to low temperatures, making cold stress a significant factor limiting its growth, especially during the bud bursting stage. To address this, an RIL population derived from a cross between cold-tolerant and cold-sensitive rice varieties was used to identify nine QTLs linked to cold tolerance under temperatures of 4 ℃, 5 °C, and 6 ℃ using a high-density genetic map. One candidate gene, LOC_Os07g44410, was identified through gene function annotation, haplotype analysis, and qRT-PCR, with two main haplotypes (Hap1 and Hap2) showing distinct phenotypic differences. qRT-PCR analysis showed that the expression level of LOC_Os07g44410 in cold tolerant lines carrying Hap1 was significantly higher than that in cold sensitive lines carrying Hap2. Hap1, associated with greater

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11754777 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature