Microenvironment-confined kinetic elucidation and implementation of a DNA nano-phage with a shielded internal computing layer.

ABSTRACT: Multiple receptor analysis-based DNA molecular computation has been developed to mitigate the off-target effect caused by nonspecific expression of cell membrane receptors. However, it is quite difficult to involve nanobodies into molecular computation with programmed recognition order because of the "always-on" response mode and the inconvenient molecular programming. Here we propose a spatial segregation-based molecular computing strategy with a shielded internal computing layer termed DNA nano-phage (DNP) to program nanobody into DNA molecular computation and build a series of kinetic models to elucidate the mechanism of microenvironment-confinement. We explain the contradiction between fast molecular diffusion and effective DNA computation using a "diffusion trap" theory and comprehens

SUBMITTER: Tang D 

PROVIDER: S-EPMC11754784 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature