Agrobacterium-mediated Cuscuta campestris transformation as a tool for understanding plant-plant interactions.

ABSTRACT: Cuscuta campestris, a stem parasitic plant, has served as a valuable model plant for the exploration of plant-plant interactions and molecular trafficking. However, a major barrier to C. campestris research is that a method to generate stable transgenic plants has not yet been developed. Here, we describe the development of a Cuscuta transformation protocol using various reporter genes (GFP, GUS, or RUBY) and morphogenic genes (CcWUS2 and CcGRF/GIF), leading to a robust protocol for Agrobacterium-mediated C. campestris transformation. The stably transformed and regenerated RUBY C. campestris plants produced haustoria, the signature organ of parasitic plants, and these were functional in forming host attachments. The locations of T-DNA integration in the parasite genome were confirmed throu

SUBMITTER: Adhikari S 

PROVIDER: S-EPMC11754947 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature