Identification of RAD51AP1 as a key gene in hepatitis B virus-associated hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is a significant global health concern, with chronic hepatitis B virus (HBV) infection being a major contributor. Understanding the mechanisms of HBV-associated HCC is crucial to improving the prognosis and developing effective treatments.

Methods

HBV-associated HCC datasets (GSE19665, GSE121248, GSE55092, GSE94660, and TCGA-LIHC) acquired from public databases were mined to identify key driver genes by differentially expressed gene analysis, weighted gene co-expression network analysis (WGCNA), followed by protein-protein interaction network analysis, Lasso-Cox regression analysis, and randomforestSRC algorithm. Then, in vitro experiments including CCK-8 assay, wound healing, and Transwell assay were performed to explore the functio

SUBMITTER: Wan M 

PROVIDER: S-EPMC11755046 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature