A screening setup to streamline <i>in vitro</i> engineered living material cultures with the host.

ABSTRACT: Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. Determining the conditions that sustain ELM performance while ensuring compatibility with ELM hosts is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through in vitro investigations. Currently, there are no standards that ensure ELM compatibility with host tissues. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential in vitro. We showed proliferation of three bacterial species encapsulated in hydrogels over ti

SUBMITTER: Desai K 

PROVIDER: S-EPMC11755081 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature