Optimized Synthesis and Stabilization of Superparamagnetic Iron Oxide Nanoparticles for Enhanced Biomolecule Adsorption.

ABSTRACT: Monodisperse and colloidally stable magnetic iron oxide nanoparticles have been developed for diverse biotechnology applications. Although promising for the adsorption of organic molecules, the low density of adsorption sites in these nanoparticles has been a significant challenge. In this study, an optimized factorial design with response surface methodology (RSM) was employed to produce small Superparamagnetic Iron Oxide Nanoparticles (SPIONs) stabilized with tetraethoxysilane (TEOS). Bovine Serum Albumin (BSA) was selected for immobilization on the surface of SPIONs to test adsorption capacity. The model was validated by correlating significant factors with experimental responses, enabling the prediction of the smallest nanoparticle size. We obtained superparamagnetic SPIONs (75.12 emu/

SUBMITTER: Juvencio Keijok W 

PROVIDER: S-EPMC11755185 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature