"Small is beautiful" - Examining reliable determination of low-abundant therapeutic antibody glycovariants.

ABSTRACT: Glycans associated with biopharmaceutical drugs play crucial roles in drug safety and efficacy, and therefore, their reliable detection and quantification is essential. Our study introduces a multi-level quantification approach for glycosylation analysis in monoclonal antibodies (mAbs), focusing on minor abundant glycovariants. Mass spectrometric data is evaluated mainly employing open-source software tools. Released N-glycan and glycopeptide data form the basis for integrating information across different structural levels up to intact glycoproteins. Comprehensive comparison showed that indeed, variations across structural levels were observed especially for minor abundant species. Utilizing modification finder (MoFi), a tool for annotating mass spectra of intact proteins, we quant

SUBMITTER: Bottinger K 

PROVIDER: S-EPMC11755342 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature