Development and Validation of a High-Throughput Quantification Method of Crown Procyanidins in Different Wines by UHPLC-Q-TOF.

ABSTRACT: Procyanidins are widely distributed in plant-derived foods, and consist of flavanol oligomers and polymers. Recently, the crown procyanidin sub-family, characterised by a unique macrocyclic structure, has been identified in grapes and wine. This study reports the development and validation of a rapid and quantitative analytical method measuring crown procyanidin concentration in red and white wines using ultra-high-performance liquid chromatography (UHPLC) coupled with a Q-TOF mass spectrometer. Validation followed international standards, demonstrating high sensitivity (LOQ = 0.033 mg/L), accuracy (recovery = 88.21% to 107.64%), repeatability (RSD = 1.99% to 11.03%), and intermediate reproducibility (RSD = 2.51% to 19.05%). Minimal matrix effects were observed, ensuring reliable and preci

SUBMITTER: Ferreira M 

PROVIDER: S-EPMC11755646 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature