Whole-Genome Sequencing of Resistance, Virulence and Regulation Genes in Extremely Resistant Strains of <i>Pseudomonas aeruginosa</i>.

ABSTRACT:

Background/objectives

Pseudomonas aeruginosa is a clinically significant opportunistic pathogen, renowned for its ability to acquire and develop diverse mechanisms of antibiotic resistance. This study examines the resistance, virulence, and regulatory mechanisms in extensively drug-resistant clinical strains of P. aeruginosa.

Methods

Antibiotic susceptibility was assessed using the Minimum Inhibitory Concentration (MIC) method, and whole-genome sequencing (WGS) was performed on the Illumina NovaSeq platform.

Results

The analysis demonstrated a higher prevalence of virulence genes compared to resistance and regulatory genes. Key virulence factors identified included secretion systems, motility, adhesion, and biofilm formation. Resistance mechanisms observ

SUBMITTER: Pajaro-Castro N 

PROVIDER: S-EPMC11755660 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature