Magnesium ions regulate the Warburg effect to promote the differentiation of enteric neural crest cells into neurons.

ABSTRACT:

Background

Understanding how enteric neural crest cells (ENCCs) differentiate into neurons is crucial for neurogenesis therapy and gastrointestinal disease research. This study explores how magnesium ions regulate the glycolytic pathway to enhance ENCCs differentiation into neurons.

Materials and methods

We used polymerase chain reaction, western blot, immunofluorescence, and multielectrode array techniques to assess magnesium ions' impact on ENCCs differentiation. Non-targeted metabolomic sequencing, cellular acidification rate, oxygen consumption, and western blot analyzed sugar metabolism changes. D-glucose-¹³C6 isotope tracing identified key glucose flux changes. Surface plasmon resonance was used to detect the binding affinity of magnesium ions with key glyco

SUBMITTER: Xu Q 

PROVIDER: S-EPMC11755793 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature