Bisecting GlcNAc modification of vesicular GAS6 regulates CAFs activation and breast cancer metastasis.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are a pivotal component of the tumor microenvironment (TME), playing key roles in tumor initiation, metastasis, and chemoresistance. While glycosylation is known to regulate various cellular processes, its impact on CAFs activation remains insufficiently explored.

Methods

We assessed the correlation between bisecting GlcNAc levels and CAFs markers (α-SMA, PDGFRA, PDGFRB) in breast cancer tissues. The effects of small extracellular vesicles (sEVs) derived from MDA-MB-231/OE^vec and MDA-MB-231/OE^MGAT3 cells on CAFs activation were examined using western blotting, transwell, and collagen contraction assays. Proteomic analysis was performed to identify dysregulated proteins in sEVs from different cell lines. Th

SUBMITTER: Li J 

PROVIDER: S-EPMC11755815 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature