Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms.

ABSTRACT:

Background

Periodontitis is not always satisfactorily treated with conventional scaling and root planing, and adjunctive use of antibiotics is required in clinical practice. Therefore, it is important for clinicians to understand the diversity and the antibiotic resistance of subgingival microbiota when exposed to different antibiotics.

Materials and methods

In this study, subgingival plaques were collected from 10 periodontitis patients and 11 periodontally healthy volunteers, and their microbiota response to selective pressure of four antibiotics (amoxicillin, metronidazole, clindamycin, and tetracycline) were evaluated through 16S rRNA gene amplicon and metagenomic sequencing analysis. Additionally, sensitive and resistant strains were isolated and cultured in vitro

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC11756365 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature