Interlaboratory comparison of an intestinal triple culture to confirm transferability and reproducibility.

ABSTRACT: The development and improvement of advanced intestinal in vitro models has received increasing attention in recent years. While the availability of relevant in vitro models is pivotal to advance the replacement and reduction of animal use in research, their robustness is a crucial determinant for intra- and interlaboratory reproducibility. We have developed a standard protocol to build a triple culture model combining two types of human intestinal epithelial cells (Caco-2, HT29-MTX-E12) and macrophages (THP-1), which was tested for transferability and reproducibility between three laboratories. The epithelial tissue barrier development and triple culture stability were investigated as well as the models' responses to the non-steroidal anti-inflammatory drug diclofenac in terms of barrier i

SUBMITTER: Kampfer AAM 

PROVIDER: S-EPMC11756443 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature