Cortical Spheroid Model for Studying the Effects of Ischemic Brain Injury.

ABSTRACT:

Purpose

Ischemic brain injury occurs when there is reduced or complete disruption of blood flow to a brain region, such as in stroke or severe traumatic brain injury. Even short interruptions can lead to devastating effects including excitotoxicity and widespread cell death. Despite many decades of research, there are still very few therapeutic options for patients suffering from brain ischemia.

Methods

We developed an in vitro brain ischemia model using our previously established 3D spheroids derived from primary postnatal rat cortex. These spheroids provide an in vivo-relevant model containing a similar cellular composition to the native cortex and a cell-synthesized extracellular matrix. This model is cost-effective, highly reproducible, and can be produced in a high-thro

SUBMITTER: McLaughlin RM 

PROVIDER: S-EPMC11756444 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature