Development and characterisation of a novel complex triple cell culture model of the human alveolar epithelial barrier.

ABSTRACT: Owing to increased pressure from ethical groups and the public to avoid unnecessary animal testing, the need for new, responsive and biologically relevant in vitro models has surged. Models of the human alveolar epithelium are of particular interest since thorough investigations into air pollution and the effects of inhaled nanoparticles and e-cigarettes are needed. The lung is a crucial organ of interest due to potential exposures to endogenous material during occupational and ambient settings. Here, an in vitro model of the alveolar barrier has been created in preparation for use in the quasi-air liquid interface (qALI) and (aerosol) air-liquid interface (ALI) exposures. The model consists of an alveolar type 1-like cell line (TT1), an alveolar type 2-like cell line (NCI-H441) and a mode

SUBMITTER: Mitchell SM 

PROVIDER: S-EPMC11756452 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature