Macro- and micro-nutrient-based multiplex stress conditions modulate in vitro tumorigenesis and aggressive behavior of breast cancer spheroids.

ABSTRACT:

Purpose

The aggressive nature of a tumor is presumably its inherent one, but different environmental cues can manipulate it in many ways. In this context, the influence of metabolic stresses on tumor behavior needs to be analyzed to understand their far-reaching implications on tumor aggression and dormancy. This work investigates different facets of the tumor, such as tumorigenic capacity, tumor phenotype, and migration, under multiple metabolic stress conditions.

Methods

Non-invasive and invasive multicellular spheroids (MTS) were created and subjected to multiple stress conditions, namely glucose, amino acid, and oxygen deprivation. Altered behavior of the MTS has been evaluated in the context of in vitro tumorigenesis, spheroid formation capacity, phenotype, mRNA profile

SUBMITTER: Gayan S 

PROVIDER: S-EPMC11756478 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature