Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii.

ABSTRACT: Sustainable chemical production from C₁ gaseous substrates, such as syngas or CO₂/H₂, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbon source and electron acceptor, while reduced inorganic species are used as the electron donor. Clostridium ljungdahlii, a model acetogen, is only capable of reducing CO₂ to acetate and ethanol, with H₂ as electron donor. In order to expand the product profile of this bacterium, five alcohol acetyltransferases (AATs) from yeast were heterologously expressed in C. ljungdahlii to evaluate its potential to produce ethyl acetate. When growing on CO₂ and H₂, up to 7.38

SUBMITTER: Boto ST 

PROVIDER: S-EPMC11756512 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature