DECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution.

ABSTRACT: Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody's target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing). This method allowed identifying patterns of epitopes recognized by monoclonal or polyclonal antibodies at single amino acid resolution and predicted cross-reactivity against the entire protein database. By applying the obtained epitope information, it has become possible to develop a new 3D immunostaining method that increases t

SUBMITTER: Matsumoto K 

PROVIDER: S-EPMC11756784 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature