Molecular determinants of terminal growth arrest induced in tumor cells by a chemotherapeutic agent.

ABSTRACT: Treatment with chemotherapy or radiation is not invariably cytotoxic to all tumor cells. Some of the cells that survive treatment recover and resume proliferation, whereas others undergo permanent growth arrest. To understand the nature of treatment-induced terminal growth arrest, colon carcinoma cells were exposed to doxorubicin, and surviving cells were separated into proliferating and growth-arrested populations. Only growth-arrested cells displayed phenotypic markers of cell senescence and failed to form colonies. Gene expression was compared between senescent and proliferating fractions of drug-treated cells by using cDNA microarray hybridization and reverse transcription-PCR. Drug-induced senescence was associated with inhibition of genes involved in cell proliferation and with coind

SUBMITTER: Chang BD 

PROVIDER: S-EPMC117570 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature