Interaction with IGF1 overrides ANXA2-mediated anti-inflammatory functions of IGFBP5 <i>in vivo</i>.

ABSTRACT:

Background

IGFBP5 is a differentially expressed gene (DEG) between M1 and M2 macrophages. This study explained why it causes opposite effects in different circumstances.

Methods

Gene expression profiles of various cell subsets were compared by mining a public database. THP-1 cells were treated by siRNAs, recombinant IGFBP5, lipopolysaccharide (LPS), picropodophyllin, IGF1 or the combinations. Clinical implication of IGFBP5 changes was investigated using rheumatoid arthritis (RA) and acute lung injury (ALI) models. IGFBP5-bound and differential proteins were identified by Liquid Chromatography Mass Spectrometry method.

Results

IGFBP5 situated in the center of a network constructed by the DEGs of M0 and M1/2 macrophages. Its expression negatively correlated to in

SUBMITTER: Fan Y 

PROVIDER: S-EPMC11757107 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature