Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells.

ABSTRACT:

Introduction

HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on the viral envelope by the CD169/Siglec-1 membrane receptor. This interaction triggers the internalization of HIV-1 within a structure known as the sac-like compartment. While the mechanism underlying sac-like compartment formation remains elusive, prior research indicates that the process is clathrin-independent and cell membrane cholesterol-dependent and involves transient disruption of cortical actin. Here, we investigate the potential involvement of massive endocytosis (MEND) in this process.

Methods

We used live cell confocal imaging to measure the dimensions and dynamics of the compartment. We assessed the role of actin and cholesterol in f

SUBMITTER: Laguia F 

PROVIDER: S-EPMC11757119 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature