Preparation and application of a <i>Brucella</i> multiepitope fusion protein based on bioinformatics and Tandem Mass Tag-based proteomics technology.

ABSTRACT:

Introduction

Brucellosis is a widespread zoonotic disease that poses a considerable challenge to global public health. Existing diagnostic methods for this condition, such as serological assays and bacterial culture, encounter difficulties due to their limited specificity and high operational complexity. Therefore, there is an urgent need for the development of enhanced diagnostic approaches for brucellosis.

Methods

Tandem mass tag (TMT) proteomic analysis was conducted on the wild-type strain Brucella abortus (B. abortus) DT21 and the vaccine strain B. abortus A19 to identify proteins with high expression levels. The proteins that exhibited high expression in the wild-type strain were selected based on the proteomic results. Subsequently, B-cell linear

SUBMITTER: Wu Q 

PROVIDER: S-EPMC11757136 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature