Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags.

ABSTRACT: Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO₂ NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@^NBAAg, Au@^4-MBAAg and Au@^DNTBAg SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON. In the immunoassay, because of the slow-photon effect of the PNC substrate, which facilitates the light coupling with SERS nanotags, the SERS signal was highly enhanced. The LODs of 8.2, 13.7 and 47.6 fg mL^-1 were achieved for simultaneous detection of OTA, AFB1 and ZON, respective

SUBMITTER: Chen R 

PROVIDER: S-EPMC11757229 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature