De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L.

ABSTRACT: Piper longum, commonly known as long pepper, is highly valued for its bioactive alkaloid piperine, which has diverse pharmaceutical and culinary applications. In this study, we used high-throughput sequencing and de novo transcriptome assembly to analyze the transcriptomes of P. longum leaves, roots, and spikes. Our dataset consisted of 173,381 high-quality transcripts, with functional annotations highlighting key pathways involved in lysine biosynthesis and secondary metabolite production. We identified 8041 simple sequence repeats (SSRs), primarily trinucleotide repeats, adding valuable genetic markers. Additionally, we uncovered 21,235 transcription factors (TFs) across 65 families, including bHLH, NAC, and MYB, which are essential in regulating metabolism. KEGG pathway analysis categor

SUBMITTER: Prasad M 

PROVIDER: S-EPMC11758021 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature