Fasciola gigantica Recombinant Abelson Tyrosine Protein Kinase (rFgAbl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells.

ABSTRACT: Fasciola gigantica can modulate host immune mechanisms through excretory-secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite-host immune interaction. To investigate the immunoregulatory function of Abl in Fasciola gigantica, we cloned and expressed the Fasciola gigantica Abl protein and assessed its effect on specific immune functions of buffalo peripheral blood mononuclear cells (PBMCs). Recombinant F. gigantica Abelson tyrosine protein kinase (rFgAbl) was expressed in Escherichia coli. Western blot analysis was performed to assess the reactivity of anti-rFgAbl antibodies with rFgAbl, serum from F. gigantica-infected buffalo, and excreti

SUBMITTER: Zhao M 

PROVIDER: S-EPMC11758316 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature