Identification of the Highly Polymorphic Prion Protein Gene (<i>PRNP</i>) in Frogs <i>(Rana dybowskii</i>).

ABSTRACT: Prion diseases are fatal neurodegenerative diseases that can be transmitted by infectious protein particles, PrP^Scs, encoded by the endogenous prion protein gene (PRNP). The origin of prion seeds is unclear, especially in non-human hosts, and this identification is pivotal to preventing the spread of prion diseases from host animals. Recently, an abnormally high amyloid propensity in prion proteins (PrPs) was found in a frog, of which the genetic variations in the PRNP gene have not been investigated. In this study, genetic polymorphisms in the PRNP gene were investigated in 194 Dybowski's frogs using polymerase chain reaction (PCR) and amplicon sequencing. We carried out in silico analyses to predict functional alterations according to non-synonymous single

SUBMITTER: Han CS 

PROVIDER: S-EPMC11758322 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature