Identification of the Bovine CSN3 Core Promoter Region and the Relationships Between CSN3 Promoter Polymorphisms and the CSN3 A and B Alleles.

ABSTRACT: Reportedly, the number of κ-casein (κ-CN) B alleles increases the proportion of κ-CN to total protein and the κ-CN content. This phenomenon is caused by single-nucleotide polymorphisms (SNPs) in the promoter region of CSN3, which encodes the B variant. Therefore, a series of 5'-deleted promoter plasmids were constructed to define the core promoter of CSN3. The promoter activity was analyzed by comparing the luciferase activity among the recombinant vectors with truncated promoters. No mutation occurred in the core promoter region (5'-ctatcgtcagatctttcctttctgtcatcttcctattggtg-3') of CSN3 in 40 cows. A 2092 bp promoter region of CSN3 was re-sequenced for detection, and nine variants were found, of which only three variants had mutation frequencies > 40%, which wer

SUBMITTER: Li W 

PROVIDER: S-EPMC11758600 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature