High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast.

ABSTRACT: Lignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However, the challenges associated with the co-utilization of xylose and glucose often hinders the efficiency of lignocellulose bioconversion. Here, we engineered yeast Ogataea polymorpha to effectively produce free fatty acids from lignocellulose. The non-oxidative branch of the pentose phosphate pathway, and the adaptive expression levels of xylose metabolic pathway genes XYL1, XYL2 and XYL3, were systematically optimized. In addition, the introduction of xylose transporter and global regulation of transcription factors achieved synchronous co-utilization of glucose and xylo

SUBMITTER: Ni X 

PROVIDER: S-EPMC11758827 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature