Iron regulatory protein 1-deficient mice exhibit hypospermatogenesis.

ABSTRACT: Imbalances in testicular iron levels are linked to compromised sperm production and male infertility. Iron regulatory proteins (IRP) 1 and 2 play crucial roles in cellular iron regulation. We investigated the role of IRP1 on spermatogenesis using Irp1-deficient mice (Irp1^-/-). Histological analysis of the testis of Irp1^-/- mice revealed hypospermatogenesis with a significant reduction in the number of elongated spermatids and daily sperm production compared to wild-type (WT) mice. Flow cytometry of germ cells from WT and Irp1^-/- mice showed reduction in spermatocytes and round and elongated spermatids in Irp1^-/- mice, which was confirmed by histological and immunofluorescence quantification. Finally, stage VIII of spermatogenesis, crucial for spe

SUBMITTER: Harrer A 

PROVIDER: S-EPMC11758943 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature