<i>E3 Siah</i> ubiquitin ligase regulates dichotomous spermatogenesis in <i>Sitotroga cerealella</i>.

ABSTRACT: Spermatogenesis in Lepidoptera holds significant importance due to its unique process of dichotomous spermatogenesis, yielding eupyrene and apyrene spermatozoa through a complex molecular mechanism. While E3 ubiquitin ligases are known to play vital roles in spermatogenesis across various processes, their functions in dichotomous spermatogenesis remain less known. We utilized the RNAi, biochemical and microscopic procedures to unravel the function of ScE3 Siah in dichotomous spermatogenesis of adult Sitotroga cerealella. In S. cerealella E3 ligase Siah predominantly expressed in adult tissues. Knockdown of ScE3 Siah leads to disruptions in testes and sperm morphology, affecting the structure of eupyrene and apyrene sperm bundles and causing defecti

SUBMITTER: Shah S 

PROVIDER: S-EPMC11759277 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature