Examining the Formalin Fixation Method for Maintaining High RNA Quality in Surgical Lung Specimens.

ABSTRACT:

Background

Multiplex genetic testing is recommended when treating nonsmall cell lung cancer. A certain percentage of test failures in RNA assays owing to poor surgical specimen quality have been documented, and fixation failure is a possible cause. At our institution, sheet-like fixation is performed to reduce fixation time. This study aimed to compare the quality of RNA from resected lung cancer specimens following different fixation methods.

Methods

Sheet-like fixation specimens (n = 15), conventional fixation specimens of the same resected lungs (n = 15), and other lung cancer specimens collected for conventional fixation and subjected to multiplex gene-panel testing (n = 22) were retrospectively examined. RNA was extracted from each specimen. RNA quality and quantity wer

SUBMITTER: Teishikata T 

PROVIDER: S-EPMC11759292 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature