Joint fluid multi-omics improves diagnostic confidence during evaluation of children with presumed septic arthritis.

ABSTRACT:

Background

An accurate diagnosis of septic versus reactive or autoimmune arthritis remains clinically challenging. A multi-omics strategy comprising metagenomic and proteomic technologies were undertaken for children diagnosed with presumed septic arthritis to advance clinical diagnoses and care for affected individuals.

Methods

Twelve children with suspected septic arthritis were prospectively enrolled to compare standard of care tests with a rapid multi-omics approach. The multi-omics combined bacterial 16S rRNA metagenomics, single cell transcriptomics, and proteomics on knee joint fluid specimens. The diagnostic value of the multi-omics was ascertained relative to standard of care culture and PCR-negative results.

Results

Ten children with suspected primary septic

SUBMITTER: Pena-Lopez Y 

PROVIDER: S-EPMC11759420 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature