Multi-assay assessment of cytotoxicity reveals multiple mechanisms of action in 3D microtissues.

ABSTRACT: Cell viability assays are an integral component of toxicology and high-throughput drug screening studies; however, many assays rely on a single biomarker of cell death which provides an incomplete assessment of cell viability. Here, we introduce an innovative approach that combines data from multiple assays using a linear mixed effects regression model and principal component analysis. We explored the cytotoxic response of various assay-treatment combinations using four assays with distinct mechanisms of action and seven different treatments across three types of microtissue cultures. The multi-assay data revealed the presence of multifaceted cellular injuries which highlight the need for multimodal approaches to better understand complex disruptions to viability. By incorporating outputs

SUBMITTER: Zein-Sabatto A 

PROVIDER: S-EPMC11759695 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature