Metabolic Activity in Human Intermuscular Adipose Tissue Directs the Response of Resident PPARγ⁺ Macrophages to Fatty Acids.

ABSTRACT: Background/Objectives: Peroxisome proliferator-activated receptor gamma (PPARγ) is a fatty acid-binding transcription activator of the adipokine chemerin. The key role of PPARγ in adipogenesis was established by reports on adipose tissue-resident macrophages that express PPARγ. The present study examined PPARγ⁺ macrophages in human skeletal muscle tissues, their response to fatty acid (FA) species, and their correlations with age, obesity, adipokine expression, and an abundance of other macrophage phenotypes. Methods: An ex vivo human skeletal muscle model with surgical specimens that were maintained without or with FAs for up to 11 days was utilized. Immunofluorescence analysis was used to detect macrophage phenotypes and mitochondrial activity. Preconfigured arra

SUBMITTER: Chen X 

PROVIDER: S-EPMC11759838 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature