Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

ABSTRACT: The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

SUBMITTER: Carter MG 

PROVIDER: S-EPMC1175992 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature