Comparative evaluation of antifungal susceptibility testing methods of invasive Candida species and detection of FKS genes mutations in caspofungin intermediate and resistant isolates.

ABSTRACT:

Background

Fungal invasive infections caused by Candida species pose a substantial public health risk with limited therapeutic options. Antifungal susceptibility testing (AFST) is necessary to optimize the therapy. The study aimed to compare different AFST methods of Candida spp. and detect FKS gene mutations among caspofungin-intermediate and resistant isolates.

Methods

A total of 60 non-replicative invasive Candida isolates recovered from various clinical samples were included. In-vitro AFST was carried out using the ATB FUNGUS 3, Vitek-2 AST-YS08, and E-test. Hotspot (HS) regions of FKS genes were sequenced for caspofungin-intermediate and resistant isolates.

Results

Candida albicans (58.3%) was the most predominant spp., followed by C. glabrata (28.3%). Based on t

SUBMITTER: ElFeky DS 

PROVIDER: S-EPMC11760087 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature