Polymeric Gluten Proteins as Climate-Resilient Markers of Quality: Can LC-MS/MS Provide Valuable Information about Spring Wheat Grown in Diverse Climates?

ABSTRACT: In this study, the impact of the varying environments, wet-cool (2017), dry-hot (2018), and fluctuating (2019), on two spring wheat genotypes, Diskett and Bumble, grown in field conditions in southern Sweden was studied. From harvested grains, polymeric gluten proteins were fractionated and collected using SE-HPLC and then analyzed with LC-MS/MS. Proteins and peptides identified through searches against the protein sequences ofTriticum aestivum (taxon 4565) from the UniProtKB database showed 7 high molecular weight glutenin subunits (HMW-GS) and 24 low molecular weight glutenin subunits (LMW-GS) with different enrichment levels for both genotypes. Glu-B1 for HMW-GS and Glu D3 and m- and s-types for LMW-GS were dominated in both genotypes, and a small proportion of α-, γ-, and ω-glia

SUBMITTER: Lama S 

PROVIDER: S-EPMC11760153 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature