Visible Light Spectroscopy of Liquid Solutes from Femto- to Attoliter Volumes Inside a Single Nanofluidic Channel.

ABSTRACT: UV-vis spectroscopy is a workhorse in analytical chemistry that finds application in life science, organic synthesis, and energy technologies like photocatalysis. In its traditional implementation with cuvettes, it requires sample volumes in the milliliter range. Here, we show how nanofluidic scattering spectroscopy (NSS), which measures visible light scattered from a single nanochannel in a spectrally resolved way, can reduce this sample volume to the attoliter range for solute concentrations in the mM regime, which corresponds to as few as 10⁵ probed molecules. The connection of the nanochannel to a microfluidic in-and-outlet system enables such measurements in continuous flow conditions, and the integrated online optical reference system ensures their long-term stability. On

SUBMITTER: Altenburger B 

PROVIDER: S-EPMC11760169 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature